Inhaltsangabe1. Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate            Brizuela Leyre and Cuvillier Olivier 2. Isoform-Selective Assays for Sphingosine Kinase Activity            Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson 3. Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry            Constantin Bode and Markus H. Gräler 4. A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule            Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi 5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples            Barbara Visentin, Gary Reynolds, and Roger Sabbadini 6. Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation            Hideru Obinata and Timothy Hla 7. High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes            William J. Valentine and Gabor Tigyi 8. Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases            Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack 9. Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm            Silvia A. Belmonte and Laila Suhaiman 10. Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis            Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii 11. Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System            Veronique E. Miron, Bryce A. Durafourt, Jack P. Antel, and Timothy E. Kennedy 12. Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells            Elisabetta Meacci, Francesca Bini, and Chiara Battistini 13. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor            Raymond C.B. Wong, Martin F. Pera, and Alice Pébay 14. Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation            Erhard Bieberich 15. Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells            He Xiaoli, Sathish Jean, and Alirio J. Melendez 16. Sphingosine-1-Phosphate Signaling in Neural Progenitors            Phillip Callihan and Shelley B. Hooks 17. Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis            Shih-Chi Su and Kayla J. Bayless

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Inhaltsangabe1. Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate             Brizuela Leyre and Cuvillier Olivier   2. Isoform-Selective Assays for Sphingosine Kinase Activity             Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson   3. Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry             Constantin Bode and Markus H. Gräler   4. A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule             Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi   5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples             Barbara Visentin, Gary Reynolds, and Roger Sabbadini   6. Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation             Hideru Obinata and Timothy Hla   7. High Throughput Assays to Measure Intracellular Ca2+ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes             William J. Valentine and Gabor Tigyi   8. Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases             Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack   9. Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm             Silvia A. Belmonte and Laila Suhaiman   10. Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis             Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii   11. Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System             Veronique E. Miron, Bryce A. Durafourt, Jack P. Antel, and Timothy E. Kennedy   12. Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells             Elisabetta Meacci, Francesca Bini, and Chiara Battistini   13. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor             Raymond C.B. Wong, Martin F. Pera, and Alice Pebay   14. Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation             Erhard Bieberich   15. Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells             He Xiaoli, Sathish Jean, and Alirio J. Melendez   16. Sphingosine-1-Phosphate Signaling in Neural Progenitors             Phillip Callihan and Shelley B. Hooks   17. Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis             Shih-Chi Su and Kayla J. Bayless