Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.

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Inhaltsangabe1. Introduction to the Immune System             Scott McComb, Aude Thiriot, Lakshmi Krishnan, and Felicity Stark   2. Immunoproteomics: Current Technology and Applications             Kelly M. Fulton and Susan M. Twine   3. Antigen Identification Using SEREX             Ugur Sahin and Özlem Türeci   4. Antigen Discovery Using Whole-Genome Phage Display Libraries             Elisa Beghetto and Nicola Gargano   5. Methods and Applications of Serological Proteome Analysis             Kelly M. Fulton, Shannon S. Martin, Lawrence Wolfraim, and Susan M. Twine   6. PreAbsorbed Immunoproteomics: A Novel Method for the Detection of Bacterial Surface Proteins             Guangjin Liu, Wei Zhang, and Chengping Lu   7. Identification of the Antigen Content of Electroimmunoprecipitates             N. Helena Beyer and Niels H.H. Heegaard   8. An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus             Kevin M. Downard   9. In Vivo Microbial Antigen Discovery (InMAD) to Identify Diagnostic Proteins and Polysaccharides that Are Circulating During Microbial Infections             Sindy J. Chaves, Kathleen Schegg, Thomas R. Kozel, and David P. AuCoin   10. ChemoEnzymatic Production of OGlycopeptides for the Detection of Serum Glycopeptide Antibodies             Alexander Nøstdal and Hans H. Wandall   11. Enrichment and Characterization of Glycopeptide Epitopes from Complex Mixtures             Luc Tessier, Kelly M. Fulton, and Susan M. Twine   12. WholeCell MALDITOF Mass Spectrometry: A Tool for Immune Cell Analysis and Characterization             Richard Ouedraogo, Julien Textoris, Aurélie Daumas, Christian Capo, and Jean-Louis Mege   13. CellBased Arrays for the Identification of Interacting Polypeptide Domains or Epitopes             Richard H. Maier, Christina J. Maier, and Kamil Önder   14. Identification and Quantitation of MHC Class II-Bound Peptides from Mouse Spleen Dendritic Cells by Immunoprecipitation and Mass Spectrometry Analysis             Leonia Bozzacco and Haiqiang Yu   15. Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification             Fei Wen and Huimin Zhao   16. Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology             Greg Harris and Wangxue Chen   17. Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis             Timothy J. Waybright, King C. Chan, Timothy D. Veenstra, and Zhen Xiao   18. Identification of Protein Biomarkers in Human Serum Using iTRAQ and Shotgun Mass Spectrometry             Theodoros A. Koutroukides, Julian A.J. Jaros, Bob Amess, Daniel Martins-de-Souza, Paul C. Guest, Hassan Rahmoune, Yishai Levin, Mike Deery, Philip D. Charles, Svenja Hester, Arnoud Groen, Andy Christoforou, Julie Howard, Nick Bond, Sabine Bahn, and Kathryn S. Lilley   19. GenomeBased Bioinformatic Prediction of Major Histocompatibility Complex (MHC) Epitopes             Simon J. Foote   20. Structure-Based Prediction of Major Histocompatibility Complex (MHC) Epitopes             Andrew J. Bordner   21. Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics             Arsalan S. Haqqani and Danica B. Stanimirovic   22. Commercial Considerations for Immunoproteomics             Scott M. Ferguson