This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Inclusive and authoritative, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations will be an invaluable resource to both basic and clinical practitioners in stem cell biology.

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Humana Press in Springer Science + Business Media
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Inhaltsangabe1. Therapeutic Mesenchymal Stromal Cells: Where We Are Headed            Patrick J. Hanley 2. The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks            Vincent C. Chen and Larry A. Couture 3. Scalable Expansion of Human Induced Pluripotent Stem Cells in Xeno-Free Microcarriers            Sara M. Badenes, Tiago G. Fernandes, Carlos A.V. Rodrigues, Maria MargaridaDiogo, and Joaquim M.S. Cabral 4. Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs            Jens Durruthy-Durruthy and Vittorio Sebastiano 5. Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging            Sébastien Sart, Fabian Calixto Bejarano, Yuanwei Yan, Samuel C. Grant, and Yan Li 6. Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells            Gema Martin-Manso and Patrick J. Hanley 7. Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions            Heba Badraiq, Liani Devito, and Dusko Ilic 8. Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use            Phuc Van Pham and Phan Kim Ngoc 9. Xeno-Free Culture of Human Periodontal Ligament Stem Cells            Oriana Trubiani and Francesca Diomede 10. GMP-Compliant Human Adipose Tissue-Derived Stromal Cells for Cellular Therapy            Hamid-Reza Aghayan, Parisa Goodarzi, and Babak Arjmand 11. cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells            Elena Veronesi, Jorge Sans Burns, Alba Murgia, Olivia Candini, Valeria Rasini, Ilenia Mastrolia, Fabio Catani, Paolo Paolucci, and Massimo Dominici 12. GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation            Bagher Larijani, Hamid-Reza Aghayan, Parisa Goodarzi, and Babak Arjmand 13. Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting            Gonçalo M.C. Rodrigues, Tiago G. Fernandes, Carlos A.V. Rodrigues, Joaquim M.S. Cabral, and Maria Margarida Diogo 14. Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems            Joana G. Carmelo, Ana Fernandes-Platzgummer, Joaquim M.S. Cabral, and Cláudia Lobato da Silva 15. Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process            Marta Serra, Livia Roseti, and Alessandra Bassi 16. Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells            Livia Roseti, Marta Serra, and Alessandra Bassi 17. Culture of Human Limbal Epithelial Stem Cells on Tenon's Fibroblast Feeder-Layers: A Translational Approach            Gaia Scafetta, Camilla Siciliano, Giacomo Frati, and Elena De Falco 18. Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements            Christiane L. Elseberg, Denise Salzig, and Peter Czermak