Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome.  Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.

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Humana Press in Springer Science + Business Media
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Inhaltsangabe1. Comprehensive Hematopoietic Stem Cell Isolation Methods            Kyle Rector, Yi Liu, and Gary Van Zant 2. Serial Transplantation of Bone Marrow to Test Self-Renewal Capacity of Hematopoietic Stem Cells In Vivo            Charusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang 3. Mouse Hematopoietic Stem Cell Transplantation            Hui Cheng, Paulina H. Liang, and Tao Cheng 4. Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells            Mladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel 5. Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces            Mitra Lavasani, Aiping Lu, Seth D. Thompson, Paul D. Robbins, Johnny Huard, and Laura J. Niedernhofer 6. Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy            Manuela Malatesta, Marzia Giagnacovo, Rosanna Cardani, Giovanni Meola, and Carlo Pellicciari 7. Cardiac Stem Cell Senescence            Daniela Cesselli, Federica D'Aurizio, Patrizia Marcon, Natascha Bergamin, Carlo Alberto Beltrami, and Antonio Paolo Beltrami 8. Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions            Sebastian Klepsch, Angelika Jamnig, Daniela Trimmel, Magdalena Schimke, Werner Kapferer, Regina Brunauer, Sarvpreet Singh, Stephan Reitinger, and Günter Lepperdinger 9. Methods for Assessing Effects of Wnt/ß-Catenin Signaling in Senescence of Mesenchymal Stem Cells            Hai-jie Wang and Yu-zhen Tan 10. Intra-Femoral Injection of Human Mesenchymal Stem Cells            Sindhu T Mohanty and Ilaria Bellantuono 11. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency            Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner 12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging            Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang 13. Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue            Mario Ricciardi, Mauro Krampera, and Marco Chilosi            Daniela Cesselli, Federica D'Aurizio, Patrizia Marcon, Natascha Bergamin, Carlo Alberto Beltrami, and Antonio Paolo Beltrami 8. Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions            Sebastian Klepsch, Angelika Jamnig, Daniela Trimmel, Magdalena Schimke, Werner Kapferer, Regina Brunauer, Sarvpreet Singh, Stephan Reitinger, and Günter Lepperdinger 9. Methods for Assessing Effects of Wnt/ß-Catenin Signaling in Senescence of Mesenchymal Stem Cells            Hai-jie Wang and Yu-zhen Tan 10. Intra-Femoral Injection of Human Mesenchymal Stem Cells            Sindhu T Mohanty and Ilaria Bellantuono 11. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency            Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner 12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging            Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang 13. Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue            Mario Ricciardi, Mauro Krampera, and Marco Chilosi            Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner 12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool